Product codeam9922 product name depc treated water ml. Safety data sheet for nucleasefree water e7431 neb. Diethyl pyrocarbonate depc is a chemical use to inactivate rnase enzymes and is sensitive to moisture and ph. Perform the depc treatment as described above, and add the amine to the autoclaved solution once the solution has cooled. Diethyl pyrocarbonate has been used in pcr reaction for. Other companies are charging thousands of dollars to set up accounts and give you access to. Gene link depc treated water and nuclease free water depc free has been treated and autoclaved water that are certified to be free of nucleases and nucleic acids. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy.
Is it okay to use water while diluting cdna or primers. Depc reacts with amine, hydroxy and thiol groups of proteins thereby inactivating rnases and other enzymes. What is the protocol of using depc in rna extraction. Sterile depc ultrapure water material safety data sheet 16. Although autoclaving does inactivate depc by causing hydrolysis of depc. For this study, we tested transcription reactions for depc inhibition effects. Nuclease free water depc free has not been treated with depc and is certified for all molecular biology applications requiring nuclease and nucleic acid free water. Ultrapure depctreated water is suitable for use with rna. Depc is also sensitive to ammonia, which causes decomposition to urethane, a possible carcinogen. These are available as convenient pdf files online at. If one does not wish to autoclave, depc treated water may be boiled at 100 c for. This molecular biology grade water from ricca chemical comes in a 1 l petg clear container and is nuclease free, protease free, sterile, and depc treated. Ambion depctreated water is certified nucleasefree and supplied in ten bottles containing 50 ml each.
All rna solutions should be prepared using at least 0. Highlights free of endo, exodeoxyribonucleases, ribonucleases, and phosphatases. Depc treated water 1 ml t7 promoter forward primer 0. Data sheet for information regarding hazards and safe handling practices. Water, rnase free, endotoxin testeddepc treated safety data sheet according to federal register vol. Diethyl pyrocarbonate depc, also called diethyl dicarbonate iupac name, is used in the laboratory to inactivate rnase enzymes in water and on laboratory utensils. Depc is highly unstable in the presence of tris buffers and decomposes rapidly into ethanol and co2. Thermo scientific deionized, diethylpyrocarbonate depc treated and 0. Show this safety data sheet to the doctor in attendance.
Water, depctreated, molecular biology grade cas 7732185 calbiochem cas 7732185 sterilefiltered water treated with diethyl pyrocarbonate depc. Download msds pdf search msds currently available for download bioworld offers depc for your research at low price. Ricca r9145000 molecular biology grade water, depc treated. Remove contact lenses, if present, after the first 5 minutes, then continue rinsing eye.
Material safety data sheet 910 clopper road gaithersburg, maryland 20878 usa. Kd medical inc depc ultra pure water, 20l depc ultra. Immediately flush eyes with large amounts of water for at least 20 minutes adequate flushing is achieved by separating eyelids with clean fingers. Open and use always depc treated water vials or bottles aseptically for all working preparations, which you cannot ignore at all. Material safety data sheet water, depc treated sc204391 hazard alert code key. Water, depc treated is a depc diethylpyrocarbonate treated and sterile filtered water. Depc treated water safety overview national diagnostics. When preparing tris buffers, treat water with depc first, and then dissolve tris to make the appropriate buffer. Water, depctreated, molecular biology grade cas 773218. Previously we used to use rnase free water depc water for all genetic experiments that is rna extraction, cdna synthesis, semipcr or rt. Also our dnasernase free distilled water is not treated with depc. Diethyl pyrocarbonate depc is an efficient, nonspecific inhibitor of rnases.
View product specific information, msds, references and buying faq. It decomposes at 155c, to ethanol and carbon dioxide in aqueous solution. Depc inactivates rnase enzymes in water by covalent modifications of the histidine residues. Shake vigorously, and allow the solution to sit overnight in a fume hood. Information in this sds is from available published sources and. Nuclease free water our nuclease free water is available as either depc treated or non depc treated water. Depc will react with primary amines and cannot be used directly to treat tris buffers. If one does not wish to autoclave, depc treated water. Ultrapure depctreated water thermo fisher scientific. Eye contact hold eye open and rinse slowly and gently with water for 15 20 minutes. Waste treatment methods the generation of waste should be avoided or minimized wherever possible. This safety data sheet was created pursuant to the requirements of. Duplicate maxiscript transcription reactions were assembled using 32putp, and the same concentration of depc treated water. Template dna was dried to completion in a vacuum centrifuge and resuspended in 0.
Water, depc treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and sirna. Product code detector pcr dna biotinylation kit 600101. Call a poison control center or doctor for treatment. Note depctreated water is not recommended for pcr or in vivo experiments. Material safety data sheet sterile depc ultrapure water. Safety summary see msds for complete information before using product. Depc has been reported to be incompatible with polycarbonate or polystyrene containers. To decontaminate glassware of rnase, the glassware may be incubated with a depc solution for 3060 minutes, and subsequently autoclaved to decompose the depc. Extreme high moderate low section 1 chemical product and company identification product name water, depc treated. It is typically used to treat water and solutions before working with easily degraded rna. Other information for research use only, not for human or veterinary clinical use.
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